Novel Fabrication Approach of a Porous Silicon Biocompatible Membrane Evaluated within a Alveolar Coculture Model
| Autor: | Jessing, Jeffrey, Vargas, Koby, Stalder, Madeline, Schreiner, Sarah, Wiens, Cooper, Williams, Marcus, Li, Yiyan, Crawford, Jerry, Hamilton, Adam, Blake, David, Mancha, Sophie |
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| Rok vydání: | 2021 |
| Předmět: |
bepress|Engineering|Nanoscience and Nanotechnology
engrXiv|Engineering|Biomedical Engineering and Bioengineering bepress|Engineering engrXiv|Engineering|Electrical and Computer Engineering|Nanotechnology Fabrication engrXiv|Engineering|Biomedical Engineering and Bioengineering|Biomedical Devices and Instrumentation engrXiv|Engineering|Materials Science and Engineering|Other Materials Science and Engineering bepress|Engineering|Biomedical Engineering and Bioengineering bepress|Engineering|Biomedical Engineering and Bioengineering|Biomedical Devices and Instrumentation bepress|Engineering|Biomedical Engineering and Bioengineering|Molecular Cellular and Tissue Engineering engrXiv|Engineering|Nanoscience and Nanotechnology bepress|Engineering|Materials Science and Engineering|Biology and Biomimetic Materials engrXiv|Engineering bepress|Engineering|Electrical and Computer Engineering bepress|Engineering|Materials Science and Engineering|Other Materials Science and Engineering engrXiv|Engineering|Materials Science and Engineering|Biology and Biomimetic Materials engrXiv|Engineering|Electrical and Computer Engineering bepress|Engineering|Electrical and Computer Engineering|Nanotechnology Fabrication engrXiv|Engineering|Biomedical Engineering and Bioengineering|Biomaterials bepress|Engineering|Materials Science and Engineering engrXiv|Engineering|Materials Science and Engineering engrXiv|Engineering|Biomedical Engineering and Bioengineering|Molecular Cellular and Tissue Engineering bepress|Engineering|Biomedical Engineering and Bioengineering|Biomaterials |
| DOI: | 10.31224/osf.io/mztyc |
| Popis: | The use of conventional in vitro and preclinical animal models often fail to properly recapitulate the complex nature of human diseases and hamper the success of translational therapies in humans [1-3] Consequently, research has moved towards organ-on-chip technology to better mimic human tissue interfaces and organ functionality. Herein, we describe a novel approach for the fabrication of a biocompatible membrane made of porous silicon (PSi) for use in organ-on-chip technology that provides key advantages when modeling complex tissue interfaces seen in vivo. By combining well-established methods in the semiconductor industry with organ-on-chip technology, we have developed a novel way of producing thin (25 μm) freestanding PSi biocompatible membranes with both nano (~15.5 nm diameter pores) and macroporous (~0.5 μm diameter pores) structures. To validate the proposed novel membrane, we chose to recapitulate the dynamic environment of the alveolar blood gas exchange interface in alveolar co-culture. Viability assays and immunofluorescence imaging indicate that human pulmonary cells remain viable on the PSi membrane during long-term culture (14 days). Interestingly, it was observed that macrophages can significantly remodel and degrade the PSi membrane substrate in culture. This degradation will allow for more intimate physiological cellular contact between cells, mimicking a true blood-gas exchange interface as observed in vivo. Broadly, we believe that this novel PSi membrane may be used in more complex organ-on-chip and lab-on-chip model systems to accurately recapitulate human anatomy and physiology to provide further insight into human disease pathology and pre-clinical response to therapeutics. |
| Databáze: | OpenAIRE |
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