Protection of non-human primates against glanders with a gold nanoparticle glycoconjugate vaccine
| Autor: | Lisa A. Morici, Richard W. Titball, Alfredo G. Torres, Christopher L. Hatcher, Chad J. Roy, Heather Vinet-Oliphant, Anthony E. Gregory |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Glycoconjugate Biology Burkholderia mallei Article Microbiology medicine Animals chemistry.chemical_classification Antigens Bacterial Vaccines Conjugate General Veterinary General Immunology and Microbiology Burkholderia thailandensis Glanders Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Virology Antibodies Bacterial Macaca mulatta Survival Analysis Bacterial vaccine Vaccination Rhesus macaque Titer Disease Models Animal Infectious Diseases chemistry Bacterial Vaccines Molecular Medicine Nanoparticles Gold Glycoconjugates |
| Popis: | The Gram-negative Burkholderia mallei is a zoonotic pathogen and the causative agent of glanders disease. Because the bacteria maintain the potential to be used as a biothreat agent, vaccine strategies are required for human glanders prophylaxis. A rhesus macaque (Macaca mulatta) model of pneumonic (inhalational) glanders was established and the protective properties of a nanoparticle glycoconjugate vaccine composed of Burkholderia thailandensis LPS conjugated to FliC was evaluated. An aerosol challenge dose of ∼1×10(4) CFU B. mallei produced mortality in 50% of naive animals (n=2/4), 2-3 days post-exposure. Although survival benefit was not observed by vaccination with a glycoconjugate glanders vaccine (p=0.42), serum LPS-specific IgG titers were significantly higher on day 80 in 3 vaccinated animals who survived compared with 3 vaccinated animals who died. Furthermore, B. mallei was isolated from multiple organs of both non-vaccinated survivors, but not from any organs of 3 vaccinated survivors at 30 days post-challenge. Taken together, this is the first time a candidate vaccine has been evaluated in a non-human primate aerosol model of glanders and represents the initial step for consideration in pre-clinical studies. |
| Databáze: | OpenAIRE |
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