Characterizing the Cellular Response to Nitrogen-Doped Carbon Nanocups
| Autor: | William S. Saunders, Amber S. Griffith, Thomas D. Zhang, Zelal Adiguzel, Seth C. Burkert, Alexander Star, Ceyda Acilan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Biocompatibility
General Chemical Engineering 02 engineering and technology Carbon nanotube Conjugated system Endocytosis Article nanotubes law.invention lcsh:Chemistry HeLa 03 medical and health sciences biocompatibility law Cytotoxic T cell General Materials Science nanocups Cytotoxicity 030304 developmental biology 0303 health sciences biology Dopant 021001 nanoscience & nanotechnology biology.organism_classification 3. Good health lcsh:QD1-999 Biophysics 0210 nano-technology |
| Zdroj: | Nanomaterials Volume 9 Issue 6 Nanomaterials, Vol 9, Iss 6, p 887 (2019) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano9060887 |
| Popis: | Carbon nanomaterials, specifically, carbon nanotubes (CNTs) have many potential applications in biology and medicine. Currently, this material has not reached its full potential for application due to the potential toxicity to mammalian cells, and the incomplete understanding of how CNTs interface with cells. The chemical composition and structural features of CNTs have been shown to directly affect their biological compatibility. The incorporation of nitrogen dopants to the graphitic lattice of CNTs results in a unique cup shaped morphology and minimal cytotoxicity in comparison to its undoped counterpart. In this study, we investigate how uniquely shaped nitrogen-doped carbon nanocups (NCNCs) interface with HeLa cells, a cervical cancer epithelial cultured cell line, and RPE-1 cells, an immortalized cultured epithelial cell line. We determined that NCNCs do not elicit a cytotoxic response in cells, and that they are uptaken via endocytosis. We have conjugated fluorescently tagged antibodies to NCNCs and shown that the protein-conjugated material is also capable of entering cells. This primes NCNCs to be a good candidate for subsequent protein modifications and applications in biological systems. |
| Databáze: | OpenAIRE |
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