Structural dynamics at the berry colour locus in Vitis vinifera L. somatic variants
| Autor: | Claudio Moser, Manna Crespan, Silvia Vezzulli, Riccardo Velasco, Daniele Migliaro, G. Munoz-Organero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Intravarietal variability Foglietto embrionale SNP Single-nucleotide polymorphism Locus (genetics) Berry SSR and SNP Horticulture Biology 01 natural sciences Chimerism DNA sequencing Caratterizzazione molecolare Cultivar Variabilità inter- e intra-varietale SSR e SNP Chimerismo Genetics Variabilità intra-varietale Genetic diversity food and beverages 04 agricultural and veterinary sciences SSR Molecular characterization Settore AGR/07 - GENETICA AGRARIA Cell layer SSRs Molecular characterisation Genetic marker 040103 agronomy & agriculture Vite 0401 agriculture forestry and fisheries Microsatellite Grapevine Inter- and intra-varietal variability 010606 plant biology & botany SNPs |
| Popis: | This study aims at the molecular characterization of somatic variants for the berry skin colour, taking advantage of a layer-specific approach and of the knowledge of the genomic sequence along the chromosome 2. The plant material consisted of 38 individuals belonging to 14 V. vinifera cultivars (cepage), encompassing the group A ('Calitor', 'Canaiolo', 'Carinena', 'Merlot', 'Rondinella', 'Grenache' and 'Tempranillo'), colourless variants from coloured wild-type, and the group B ('Baresana', 'Chasselas', 'Italia', 'Malvasia di Candia aromatica', 'Moscato bianco', 'Sauvignon' and 'Sultanina'), coloured variants from non-coloured wild-type. A set of 11 reference SSR markers was used to confirm the varietal identification. In order to perform the genetic characterization at the berry colour locus, 10 SSR markers, 6 SNP regions, and 1 SCAR marker (Gret1) distributed along the chromosome 2 were analysed. The major findings of this study revealed a structural dynamics along the chromosome 2 both at inter- and intra-varietal level within the group A. In fact, a deletion of different extent and position was detected in the colourless variants at the inner layer level. Within group B, instead, the coloured variants could be distinguished mostly for the partial Gret1 retrotransposon excision from the VvMybA1 promoter. In conclusion, this study allows the discrimination of somatic variants and suggests the occurrence of different evolutionary models both among and within grapevine cultivars. |
| Databáze: | OpenAIRE |
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