12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka
| Autor: | Michael A. Matthias, Dinesha Jayasundara, Chandika D. Gamage, Jean-Francois Mariet, Senanayake A. M. Kularatne, Indika Senavirathna, Suneth Agampodi, Janith Warnasekara, Joseph M. Vinetz, Sisira Siribaddana, Mathieu Picardeau |
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| Přispěvatelé: | Rajarata University of Sri-Lanka (RUSL), University of Peradeniya, Yale School of Medicine [New Haven, Connecticut] (YSM), Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris] (IP), This work was supported by the United States Public Health Service by grant U19AI115658 (JV, MM, SA) from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., We would like to thank Ms. Thilakanjali Gamage, Mr. K.M.R. Premathilaka, Mr. S.K. Senevir- athna, and Mr. Milinda Perera for technical assistance, Mr. Shalka Srimantha and Ms. Chamila Kappagoda for culture maintenance and laboratory support, and Dr. Muditha Abeykoon, Dr. Chamida Wickramasinghe, and Dr. Shanika Gamage for additional culture collections. We also thank all the physicians and healthcare staff in the various participating hospitals, team of core facility P2M (Institut Pasteur, Mutualized Platform for Microbiology) for genomic sequencing and members of the National Reference Center for Leptospirosis (Institut Pasteur) for technical assistance with the cultures of Leptospira. |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bacterial Diseases Male Veterinary medicine Range (biology) Epidemiology Nosocomial Infections RC955-962 Disease Pathology and Laboratory Medicine Polymerase Chain Reaction Zoonotic disease Geographical locations 0302 clinical medicine Medical Conditions Arctic medicine. Tropical medicine Zoonoses Medicine and Health Sciences Clinical severity Prospective Studies Pathogen Leptospira Geography High-Throughput Nucleotide Sequencing Genomics Infectious Disease Epidemiology Acute Kidney Injury Middle Aged Leptospirosis Bacterial Pathogens Bacterial Typing Techniques Leptospira Interrogans Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Medical Microbiology Cardiovascular Diseases Female Pathogens Public aspects of medicine RA1-1270 Leptospira interrogans Research Article Neglected Tropical Diseases Adult DNA Bacterial medicine.medical_specialty Asia 030231 tropical medicine Biology Microbiology 03 medical and health sciences Agglutination Tests medicine Genetics Animals Humans Epidemics Microbial Pathogens Sri Lanka Bacteria Public Health Environmental and Occupational Health Organisms Biology and Life Sciences Correction medicine.disease biology.organism_classification Tropical Diseases 030104 developmental biology Typing methods Sri lanka People and places |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, 2021, 15 (3), pp.e0009272. ⟨10.1371/journal.pntd.0009272⟩ PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009272 (2021) |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Leptospirosis is a ubiquitous zoonotic disease and a major clinical challenge owing to the multitude of clinical presentations and manifestations that are possibly attributable to the diversity of Leptospira, the understanding of which is key to study the epidemiology of this emerging global disease threat. Sri Lanka is a hotspot for leptospirosis with high levels of endemicity as well as annual epidemics. We carried out a prospective study of Leptospira diversity in Sri Lanka, covering the full range of climatic zones, geography, and clinical severity. Samples were collected for leptospiral culture from 1,192 patients from 15 of 25 districts in Sri Lanka over two and half years. Twenty-five isolates belonging to four pathogenic Leptospira species were identified: L. interrogans, L. borgpetersenii, L. weilii, and L. kirschneri. At least six serogroups were identified among the isolates: Autumnalis (6), Pyrogenes (4), Icterohaemorrhagiae (2), Celledoni (1), Grippotyphosa (2) and Bataviae (1). Seven isolates did not agglutinate using available antisera panels, suggesting new serogroups. Isolates were sequenced using an Illumina platform. These data add 25 new core genome sequence types and were clustered in 15 clonal groups, including 12 new clonal groups. L. borgpetersenii was found only in the dry zone and L. weilii only in the wet zone. Acute kidney injury and cardiovascular involvement were seen only with L. interrogans infections. Thrombocytopenia and liver impairment were seen in both L. interrogans and L. borgpetersenii infections. The inadequate sensitivity of culture isolation to identify infecting Leptospira species underscores the need for culture-independent typing methods for Leptospira. Author summary There is a huge diversity in pathogenic Leptospira species worldwide, and our knowledge of the currently circulating species is deficient owing to limited isolation and identification of Leptospira species from endemic countries. This prospective study reveals the wide pathogen diversity that causes human leptospirosis in Sri Lanka, representing four species, more than six serogroups, and fifteen clonal groups. Further, the different geographic and climatic zone distributions and clinical manifestations observed underscores the need for prospective studies to expand the molecular epidemiological approaches to combat leptospirosis. |
| Databáze: | OpenAIRE |
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