Biochemical and Spectroscopic Observation of Mn(II) Sequestration from Bacterial Mn(II) Transport Machinery by Calprotectin
| Autor: | Elizabeth M. Nolan, Derek M. Gagnon, Yu Gu, R. David Britt, Toshiki G. Nakashige, Megan Brunjes Brophy, Rose C. Hadley |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Life on Land 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Oligomer Article Catalysis S100A9 S100A8 law.invention Metal 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry law medicine Extracellular 2.2 Factors relating to the physical environment Aetiology Electron paramagnetic resonance Manganese Electron Spin Resonance Spectroscopy General Chemistry 0104 chemical sciences Streptococcus pneumoniae Emerging Infectious Diseases Infectious Diseases 030104 developmental biology chemistry visual_art Chemical Sciences visual_art.visual_art_medium Calprotectin Infection Leukocyte L1 Antigen Complex |
| Zdroj: | Journal of the American Chemical Society, vol 140, iss 1 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.7b11207 |
| Popis: | Human calprotectin (CP, S100A8/S100A9 oligomer) is a metal-sequestering host-defense protein that prevents bacterial acquisition of Mn(II). In this work, we investigate Mn(II) competition between CP and two solute-binding proteins that Staphylococcus aureus and Streptococcus pneumoniae, Gram-positive bacterial pathogens of significant clinical concern, use to obtain Mn(II) when infecting a host. Biochemical and electron paramagnetic resonance (EPR) spectroscopic analyses demonstrate that CP outcompetes staphylococcal MntC and streptococcal PsaA for Mn(II). This behavior requires the presence of excess Ca(II) ions, which enhance the Mn(II) affinity of CP. This report presents new spectroscopic evaluation of two Mn(II) proteins important for bacterial pathogenesis, direct observation of Mn(II) transfer from the bacterial Mn(II) acquisition proteins to CP, and molecular-level insight into the extracellular battle for metal nutrients that occurs during infection. |
| Databáze: | OpenAIRE |
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