Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato

Autor: Kashyap, Anurag, Jiménez‐Jiménez, Álvaro Luis, Zhang, Weiqi, Capellades, Montserrat, Srinivasan, Sumithra, Laromaine, Anna, Serra, Olga, Figueras, Mercè, Rencoret, Jorge, Gutiérrez, Ana, Valls, Marc, Coll, Nuria S.
Přispěvatelé: Agencia Estatal de Investigación, Ministerio de Ciencia e Innovación (España), Fundación Severo Ochoa, Consejo Superior de Investigaciones Científicas (España), Generalitat de Catalunya, European Commission, Kashyap, Anurag, Jiménez, Álvaro, Zhang, Weiqi, Capellades, Montserrat, Srinivasan, Sumithra Y., Laromaine, Anna, Serra, Olga, Figueras, Mercè, Rencoret, Jorge, Gutiérrez Suárez, Ana, Valls Matheu, Marc, Sánchez-Coll, Núria, Kashyap, Anurag [0000-0003-2622-8209], Jiménez, Álvaro [0000-0002-9406-4595], Zhang, Weiqi [0000-0002-2535-1398], Capellades, Montserrat [0000-0001-9514-2885], Srinivasan, Sumithra Y. [0000-0002-0473-9801], Laromaine, Anna [0000-0002-4764-0780], Serra, Olga [0000-0002-1678-0932], Figueras, Mercè [0000-0002-6288-1830], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Valls Matheu, Marc [0000-0003-2312-0091], Sánchez-Coll, Núria [0000-0002-8889-0399]
Rok vydání: 2022
Předmět:
Zdroj: New Phytologist, 2022, vol. 234, núm. p. 1411-1429
Articles publicats (D-B)
DUGiDocs – Universitat de Girona
instname
Digital.CSIC. Repositorio Institucional del CSIC
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Popis: 19 páginas.- 9 figuras.- referencias
Tomato varieties resistant to the bacterial wilt pathogen Ralstonia solanacearum have the ability to restrict bacterial movement in the plant. Inducible vascular cell wall reinforcements seem to play a key role in confining R. solanacearum into the xylem vasculature of resistant tomato. However, the type of compounds involved in such vascular physico-chemical barriers remain understudied, while being a key component of resistance. Here we use a combination of histological and live-imaging techniques, together with spectroscopy and gene expression analysis to understand the nature of R. solanacearum-induced formation of vascular coatings in resistant tomato. We describe that resistant tomato specifically responds to infection by assembling a vascular structural barrier formed by a ligno-suberin coating and tyramine-derived hydroxycinnamic acid amides. Further, we show that overexpressing genes of the ligno-suberin pathway in a commercial susceptible variety of tomato restricts R. solanacearum movement inside the plant and slows disease progression, enhancing resistance to the pathogen. We propose that the induced barrier in resistant plants does not only restrict the movement of the pathogen, but may also prevent cell wall degradation by the pathogen and confer anti-microbial properties, effectively contributing to resistance.
Research is funded by MCIN/AEI/10.13039/501100011033 (NSC, MV), MCIN/AEI/PID2019-110330GB-C21 (MF, OS), MCIN/AEI/PID2020-118968RBI00 (JR), through the ‘Severo Ochoa Programme for Centres of Excellence in R&D’ (SEV-2015-0533, CEX2019-000917 and CEX2019-000902-S funded by MCIN/AEI/ 10.13039/501100011033), and by the Spanish National Research Council (CISC) pie-201620E081 (JR, AG) and the Generalitat de Catalunya (2017SGR765 grant). AK is the recipient of a Netaji Subhas – Indian Council of Agricultural Research International Fellowship. SS acknowledges financial support from DOC-FAM, European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement no. 754397. This work was also supported by the CERCA Program/Generalitat de Catalunya.
Databáze: OpenAIRE
Externí odkaz: