Lack of regio- and stereospecificity in oxidation of (+) camphor by Streptomyces griseus enriched in cytochrome P-450soy
| Autor: | F. Sima Sariaslani, Lawrence R. McGee, Fulton G. Kitson, Michael K. Trower |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytochrome
Stereochemistry Metabolite Flour Biophysics Biochemistry chemistry.chemical_compound Camphor Stereospecificity Cytochrome P-450 Enzyme System Oxidoreductase Molecular Biology Ferredoxin chemistry.chemical_classification biology Streptomyces griseus Stereoisomerism Cell Biology biology.organism_classification chemistry biology.protein Spinach Soybeans Oxidoreductases Oxidation-Reduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 170:456-461 |
| ISSN: | 0006-291X |
| Popis: | Oxidation of (+) camphor by cytochrome P-450soy-enriched intact cells of Streptomyces griseus resulted in the formation of one major and several minor metabolites. The minor metabolites were identified as 3-endo-hydroxycamphor (2%), 5-endo-hydroxycamphor (7%), 5-exo-hydroxycamphor (9%), 2,5-diketobornane (2%), and camphorquinone (3%). The major metabolite was isolated and conclusively identified as 6-endo-hydroxycamphor (60%). When supplemented with NADPH, spinach ferredoxin:NADP oxidoreductase and spinach ferredoxin, homogeneous preparations of cytochrome P-450soy oxidized camphor to a mixture of 3-endo-, 5-endo-, 5-exo-and 6-endo-hydroxycamphor. The data presented indicates that cytochrome P-450soy resembles its mammalian counterparts in its lack of regio- and stereospecificity in camphor oxidation. |
| Databáze: | OpenAIRE |
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