Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer
| Autor: | Isabel Poschke, J. de Boniface, J. Schmidt-Mende, Rolf Kiessling, M. Miyan |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Oncology CA15-3 medicine.medical_specialty Pathology Molecular subtypes T-Lymphocytes T cell Breast Neoplasms Regulatory T-lymphocytes Tumor-infiltrating lymphocytes General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Lymphocytes Tumor-Infiltrating 0302 clinical medicine Breast cancer Antigens CD Internal medicine medicine Humans Lymphocyte Count Aged Aged 80 and over Medicine(all) Biochemistry Genetics and Molecular Biology(all) business.industry Research Cytotoxic T-lymphocytes FOXP3 General Medicine Middle Aged Sentinel node Prognosis medicine.disease Immunohistochemistry Phenotype medicine.anatomical_structure 030220 oncology & carcinogenesis Female business Biomarkers CD8 030215 immunology |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| DOI: | 10.1186/s12967-016-0983-9 |
| Popis: | Background Molecular subtypes of breast cancer and presence of tumor-infiltrating immune cells have both been implicated as important predictive and prognostic factors for improved risk stratification and treatment individualization of breast cancer patients. Their association, however, has not been studied in detail. The aim of this study was to evaluate the expression of the T cell markers CD8, FoxP3, CD3 and ζ-chain in molecular subtypes of the invasive margin and tumor center of breast cancer and corresponding sentinel nodes and to deduct prognostic information from these findings. Methods Tumor and sentinel node sections from 177 patients with primary, invasive, unilateral early-stage breast cancer were stained by immunohistochemistry and T-cell phenotypes quantified manually. Clinical data were collected from medical records. Results The degree of T-cell infiltration and expression of all markers differed significantly among the molecular subtypes, being highest in non-luminal, more aggressive tumors: more T-cell infiltration and higher expression of all markers were associated with hormone receptor negativity, higher proliferation and higher histological grades, but also with larger tumor size. Basal-like tumors, and most remarkably their tumor centers, hosted the highest number of FoxP3+ T-cells with an unfavorable ratio to cytotoxic CD8+ T-cells. T-cell infiltration was generally higher in the invasive margin than the tumor center. A scoring system based on densities of CD3 and CD8 could significantly separate molecular subtypes (p |
| Databáze: | OpenAIRE |
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