Application of microbial assay for risk assessment biotest in evaluation of toxicity of human and veterinary antibiotics
| Autor: | K. Wadhia, J. Sawicki, Agnieszka I. Piotrowicz-Cieślak, G. Nałȩcz-Jawecki, Barbara Adomas |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiological Techniques
medicine.drug_class Health Toxicology and Mutagenesis Microorganism Antibiotics Microbial metabolism Management Monitoring Policy and Law Biology Quinolones Toxicology Risk Assessment Microbiology chemistry.chemical_compound Toxicity Tests medicine Ecotoxicology Bioassay Animals Humans Strain (chemistry) Bacteria Dose-Response Relationship Drug Veterinary Drugs General Medicine Anti-Bacterial Agents chemistry Tetracyclines Toxicity Biological Assay Growth inhibition Water Microbiology |
| Zdroj: | Environmental toxicology. 25(5) |
| ISSN: | 1522-7278 |
| Popis: | The microbial assay for risk assessment (MARA) is a multispecies, growth inhibition microplate toxicity test with 11 microorganisms individually lyophilized in microplate wells. The microbial species representing wide diversity, generated 11 microbial toxic concentration (MTC) values were obtained giving a unique "toxic fingerprint" profile of the test sample. The toxicity of 14 antibiotics was evaluated with the MARA test. The fingerprints for each group of antibiotics differ, indicating a particular toxic profile. Tetracyclines were the most toxic antibacterials with the minimum MTC value of 3.6 μg L(-1). In the group of tetracyclines the order of the three most sensitive microbial strains was the same 2 > 6 > 7. Quinolones affected the most sensitive strain(s) at concentrations of 12-75 μg L(-1). The MARA bioassay exhibited different spectra of toxic responses to microbial strains for the first and second generation quinolones. However, for first generation quinolones strain 6 was substantially more sensitive than the other microorganisms, while second generation quinolones were most toxic to strain 3, followed by 6 then 4. In this instance, the three strains belong to two different phylogenetic groups-strain 3 is γ-proteotype and strains 4 and 6 are β-proteotype. |
| Databáze: | OpenAIRE |
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