Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane
| Autor: | Cornelia Schroeder, Kai Simons, C A Lingwood, Julio L. Sampaio, Beth Binnington, Lucie Kalvodova, Dirk Lindemann, Severino Urban, Jean-Marc Verbavatz, Mathias J. Gerl, Andrej Shevchenko |
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| Rok vydání: | 2012 |
| Předmět: |
Membrane lipids
Orthomyxoviridae Biology Mass Spectrometry Cell Line Cell membrane Membrane Lipids Dogs Viral envelope Report medicine Animals Research Articles Sphingolipids Budding Cell Membrane Membrane raft Cell Biology Apical membrane biology.organism_classification Cell biology Cholesterol Membrane medicine.anatomical_structure lipids (amino acids peptides and proteins) |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Analysis of the lipid composition of influenza virus–infected cells provides support for the membrane raft-based biogenesis model. The influenza virus (IFV) acquires its envelope by budding from host cell plasma membranes. Using quantitative shotgun mass spectrometry, we determined the lipidomes of the host Madin–Darby canine kidney cell, its apical membrane, and the IFV budding from it. We found the apical membrane to be enriched in sphingolipids (SPs) and cholesterol, whereas glycerophospholipids were reduced, and storage lipids were depleted compared with the whole-cell membranes. The virus membrane exhibited a further enrichment of SPs and cholesterol compared with the donor membrane at the expense of phosphatidylcholines. Our data are consistent with and extend existing models of membrane raft-based biogenesis of the apical membrane and IFV envelope. |
| Databáze: | OpenAIRE |
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