Pro-neurogenic effects of andrographolide on RSC96 Schwann cells in vitro
| Autor: | Jinmin Zhao, Kun Zhang, Fuben Xu, Li Zheng, Peizhen Lv, Huayu Wu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Cell Survival proliferation Cell Gene Expression Schwann cell S100 Calcium Binding Protein beta Subunit Ciliary neurotrophic factor Biochemistry Cell Line 03 medical and health sciences 0302 clinical medicine Neurotrophic factors Genetics medicine Animals Ciliary Neurotrophic Factor RNA Messenger Viability assay Molecular Biology Cell Proliferation biology Cell growth Brain-Derived Neurotrophic Factor andrographolide Articles RSC96 Schwann cells Rats Neuroprotective Agents 030104 developmental biology medicine.anatomical_structure Oncology Cell culture peripheral nerve Peripheral nerve injury Cancer research biology.protein Molecular Medicine Andrographis Schwann Cells Diterpenes Corrigendum 030217 neurology & neurosurgery |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2016.5717 |
| Popis: | Nerve regeneration remains a challenge to the treatment of peripheral nerve injury. Andrographolide (Andro) is the main active constituent of Andrographis paniculata, which has been applied in the treatment of several diseases, including inflammation, in ancient China. Andro has been reported to facilitate the reduction of edema and to exert analgesic effects in the treatment of various diseases. These findings suggest that Andro may be considered a promising anti-inflammatory agent that may suppress destruction and accelerate proliferation of Schwann cells following peripheral nerve injury. In the present study, the effects of Andro on RSC96 cells were investigated in vitro. The RSC96 cell line is a spontaneously immortalized rat Schwann cell line, which was originally derived from a long-term culture of rat primary Schwann cells. RSC96 cells were treated with a range of 0 to 50 µM Andro prior to the MTT assay. Cell proliferation, morphology, synthesis and nerve-specific gene expression were performed to detect the effect of Andro on RSC96 cells. The results of the present study demonstrated that the recommended doses of Andro ranged between 0.78 and 12.5 µM, among which the most obvious response was observed when used at 3.125 µM (P |
| Databáze: | OpenAIRE |
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