Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis

Autor:	Henry R. Bourne, Guy Servant, Orion D. Weiner, John W. Sedat, Paul Herzmark, Tamas Balla
Rok vydání:	2000
Předmět:	Pleckstrin homology domain Multidisciplinary Proto-Oncogene Proteins c-akt G protein Cell polarity Chemotaxis Biology Signal transduction Protein kinase A Molecular biology Protein kinase B Article Cell biology
Zdroj:	Science. 287:1037-1040
ISSN:	1095-9203 0036-8075
Popis:	Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile , indicating that it requires activity of one or more Rho guanosine triphosphatases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31b39026fe53acc74ad87d0c7470f54c https://doi.org/10.1126/science.287.5455.1037 Zobrazit plný text záznamu Plný text ve formátu PDF