ADrosophilaLexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research
| Autor: | Deanna Havey, Uriel Tayvah, Lydia Stahr, Anika Ayyar, Theresa Clark, Grace E Kim, Lutz Kockel, Townley Chisholm, Ja-Hon Wang, Samuel Han, Diane Lee, Dana Tung, Anne E. Rankin, Jiapei Chen, Eun Soo Jackie Kim, Christina Savvides, Seung K. Kim, Elle MacAlpine, Sarah Xiao, Kaelina Lombardo, Madeline Logan, Sangbin Park, Hansen Shi, Sydni M. Topper, Ida Piyale, Emma Herold, Vahid Fazel-Rezai, Flora Wang, Lutfi Huq, Ravi Jagadeesan, Cheryl Rotondo, Trang Duong |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transposable element Drosophila neuro-endocrine system enhancer trap Gene Expression Computational biology QH426-470 Investigations 03 medical and health sciences Genes Reporter Genetics medicine Animals Drosophila Proteins Enhancer trap Molecular Biology Genetics (clinical) Neuroendocrine cell biology Research Chromosome Mapping Gene Expression Regulation Developmental biology.organism_classification Immunohistochemistry LexA-LexAop binary expression system Mutagenesis Insertional Drosophila melanogaster Enhancer Elements Genetic 030104 developmental biology medicine.anatomical_structure Organ Specificity Larva bacteria Drosophila Repressor lexA Stem cell Developmental biology Drosophila Protein Developmental Biology |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3017-3026 (2016) |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.116.031229 |
| Popis: | Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila. However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals. |
| Databáze: | OpenAIRE |
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