Pattern of circulating SARS‐CoV‐2‐specific antibody‐secreting and memory B‐cell generation in patients with acute COVID‐19
| Autor: | Grigory A. Efimov, Maria G. Byazrova, Anna B. Spiridonova, G. M. Yusubalieva, Konstantin Baranov, Dmitriy Mazurov, Alexander Filatov, Vladimir P. Baklaushev |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
antibody‐secreting cell lcsh:Immunologic diseases. Allergy memory B cell Immunology medicine.disease_cause virus‐neutralising antibodies Virus SARS‐CoV‐2 Flow cytometry COVID-19 plasmablast antibody-secreting cell virus-neutralising antibodies SARS-CoV-2 03 medical and health sciences 0302 clinical medicine Antigen medicine Immunology and Allergy Memory B cell General Nursing Coronavirus CD40 biology medicine.diagnostic_test ELISPOT 030104 developmental biology Humoral immunity biology.protein Original Article lcsh:RC581-607 030215 immunology |
| Zdroj: | Clinical & Translational Immunology, Vol 10, Iss 2, Pp n/a-n/a (2021) Clinical & Translational Immunology Clinical & Translational Immunology, 10(2):e1245 |
| ISSN: | 2050-0068 |
| Popis: | Objectives To predict the spread of coronavirus disease (COVID‐19), information regarding the immunological memory for disease‐specific antigens is necessary. The possibility of reinfection, as well as the efficacy of vaccines for COVID‐19 that are currently under development, will largely depend on the quality and longevity of immunological memory in patients. To elucidate the process of humoral immunity development, we analysed the generation of plasmablasts and virus receptor‐binding domain (RBD)‐specific memory B (Bmem) cells in patients during the acute phase of COVID‐19. Methods The frequencies of RBD‐binding plasmablasts and RBD‐specific antibody‐secreting cells (ASCs) in the peripheral blood samples collected from patients with COVID‐19 were measured using flow cytometry and the ELISpot assay. Results The acute phase of COVID‐19 was characterised by the transient appearance of total as well as RBD‐binding plasmablasts. ELISpot analysis indicated that most patients exhibited a spontaneous secretion of RBD‐specific ASCs in the circulation with good correlation between the IgG and IgM subsets. IL‐21/CD40L stimulation of purified B cells induced the activation and proliferation of Bmem cells, which led to the generation of plasmablast phenotypic cells as well as RBD‐specific ASCs. No correlation was observed between the frequency of Bmem cell‐derived and spontaneous ASCs, suggesting that the two types of ASCs were weakly associated with each other. Conclusion Our findings reveal that SARS‐CoV‐2‐specific Bmem cells are generated during the acute phase of COVID‐19. These findings can serve as a basis for further studies on the longevity of SARS‐CoV‐2‐specific B‐cell memory. In this study, we evaluated the frequencies of plasmablasts and memory B cells that specifically target the SARS‐CoV‐2 receptor binding domain. Our findings reveal that SARS‐CoV‐2‐specific memory B cells are generated during the acute phase of COVID‐19. These findings can serve as a basis for further studies on the longevity of SARS‐CoV‐2‐specific B cell memory. |
| Databáze: | OpenAIRE |
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