Prevalence of beta-exotoxin, diarrhoeal toxin and specific delta-endotoxin in natural isolates of Bacillus thuringiensis
| Autor: | Alka Vaid, Alistair H. Bishop, Michela Perani |
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| Rok vydání: | 1998 |
| Předmět: |
Diarrhea
Adenosine Bacterial Toxins Bacillus cereus Bacillus thuringiensis Bacillus Enterotoxin medicine.disease_cause Microbiology Enterotoxins Hemolysin Proteins Bacterial Proteins Genetics medicine Protein Precursors Molecular Biology Bacillaceae biology Bacillus thuringiensis Toxins Toxin Sugar Acids biology.organism_classification Bacillales Endotoxins Exotoxin |
| Zdroj: | FEMS microbiology letters. 160(1) |
| ISSN: | 0378-1097 |
| Popis: | Of newly isolated colonies with the appearance of Bacillus thuringiensis, 47.5% were found to produce the parasporal crystals characteristic of this species. These positive isolates were screened using the polymerase chain reaction for their possession of a gene encoding a specific protoxin type, CryIB. Strains with and without this gene were screened for their ability to produce beta-exotoxin and Bacillus cereus-type enterotoxin. It was found that 35% of the isolates possessed the cryIB gene; of these 83% also produced enterotoxin and 58% produced beta-exotoxin. No statistical significance was found for linkage between any of these characteristics. The probability, therefore, of isolating a strain of B. thuringiensis which specifically possessed the cryIB gene but did not produce either of the other, undesired, toxins, from the soil sample used, was 1.2%. |
| Databáze: | OpenAIRE |
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