Novel Functional Role of NK3R Expression in the Potentiating Effects on Somatolactin α Autoregulation in grass carp pituitary cells
| Autor: | Anderson On-Lam Wong, Guangfu Hu, Mulan He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fish Proteins medicine.medical_specialty Carps Neurokinin B Stimulation Article 03 medical and health sciences Insulin-Like Growth Factor II Internal medicine Gene expression medicine Cyclic AMP Animals Homeostasis Secretion RNA Messenger Insulin-Like Growth Factor I Carp Receptor Protein kinase C Cells Cultured Protein Kinase C Sirolimus Messenger RNA Multidisciplinary biology Chemistry Receptors Neurokinin-3 biology.organism_classification Cyclic AMP-Dependent Protein Kinases Recombinant Proteins Grass carp Up-Regulation Pituitary Hormones 030104 developmental biology Endocrinology Pituitary Gland Type C Phospholipases Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep36102 |
| Popis: | In our previous study, NKB/NK3R system has been shown to act at the pituitary level to up-regulate SLα synthesis and secretion in grass carp. However, whether NK3R expression can serve as a regulatory target at the pituitary level and contribute to NKB interactions with other SLα regulators is still unclear. In current study, using grass carp pituitary cells as a model, we have a novel finding that co-treatment of SLα/SLβ with carp TAC3 gene products, could induce a noticeable enhancement in SLα mRNA expression and these potentiating effects occurred with a parallel rise in NK3R transcript level after SLα/SLβ treatment. Interestingly, the stimulatory effects of SLα/SLβ on NK3R gene expression could be further potentiated by co-treatment with IGF-I/-II and simultaneous exposure of carp pituitary cells to SLα/SLβ and IGF-I/-II in the presence of TAC3 gene products was found to markedly elevated SLα mRNA expression (20 fold increase) and this synergistic stimulation was mediated by cAMP/PKA-, PLC/PKC- and Ca2+ -dependent cascades functionally coupled with NK3R activation. These findings suggest that local release of SLα via functional interactions with IGF-I/-II and TAC3/NK3R system may constitute a potent stimulatory signal for SLα gene expression in the carp pituitary via up-regulation of NK3R expression. |
| Databáze: | OpenAIRE |
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