| Popis: |
Cell migration is a crucial phenomenon for many biological processes, including embryonic development, wound healing, cancer metastasis, and inflammation. Dictyostelium discoidium is model organism of cell migration and attracted toward cAMP. In D. discoidium cells, chemotactic signaling components such as PI(3,4,5)P3 and PTEN are polarized without external cues. The mutually exclusive distribution of PI(3,4,5)P3 and PTEN are self-organized on plasma membrane independently on actin dynamics. Each domain propagates as traveling waves. Microtubule network is a key component on cell polarization in several types of cell including D. discoidium. Because vesicle transportation is involved in phosphatidylinositol (PtdIns) metabolic system, microtubule is potentially able to control the dynamics of PI(3,4,5)P3 and PTEN on plasma membrane. However, the relationship between PtdIns dynamics and microtubule network is not unclear. We examined the influence of microtubule depolymerize drugs on PtdIns dynamics and revealed that these drugs had influence on domain formation of PI(3,4,5)P3 and delayed the period of traveling PI(3,4,5)P3 wave. Based on the experiments and computational simulation, we are going to clarify the relationship between the period of traveling PI(3,4,5)P3 wave and the spontaneous cell motility. We will also report the results of single molecule imaging analysis to investigate the relationship between molecular behavior of PTEN and microtubule network dynamics. |
