Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices
| Autor: | Manuel López-Rivadulla, Angelines Cruz, María Cobo-Golpe, Elena Lendoiro, Ana de-Castro-Ríos, Mario Páramo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_treatment
Clinical Biochemistry Pharmaceutical Science 01 natural sciences Analytical Chemistry Levomepromazine chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery medicine Humans Solid phase extraction Antipsychotic Spectroscopy Dichloromethane Detection limit Chromatography integumentary system 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) 0104 chemical sciences Substance Abuse Detection medicine.anatomical_structure Nails Nail (anatomy) Antipsychotic Agents Chromatography Liquid Hair medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 189:113443 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2020.113443 |
| Popis: | Nail samples are an alternative to hair for long-term monitoring of drug use, although there are a limited number of studies about its applicability. This study presents the development and validation of a LC-MS/MS method for the determination of five antipsychotic drugs (clozapine, haloperidol, levomepromazine, olanzapine and quetiapine) in nail and in hair samples. Samples were washed with dichloromethane, pulverized with a ball mill, and incubated in water:acetonitrile (50:50 v/v) with horizontal agitation for 90 min. Then, samples were purified by solid phase extraction with OASIS MCX cartridges and analysed by LC-MS/MS. The analytical method was fully validated in nails and in hair, including: limits of detection (2.5 pg/mg and 2.5-10 pg/mg, respectively), limits of quantification (LOQ) (10 pg/mg and 10-20 pg/mg, respectively), linearity (LOQ to 10,000 pg/mg), selectivity (no endogenous or exogenous interferences), accuracy (97.4-106.9% and 97.3-108.2%, respectively), imprecision ( |
| Databáze: | OpenAIRE |
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