Effects of agarose mould compliance and surface roughness on self-assembled meniscus-shaped constructs
| Autor: | Regis A. James, Najmuddin J. Gunja, Daniel J. Huey, Kyriacos A. Athanasiou |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cartilage
Articular Scaffold Materials science Surface Properties Biomedical Engineering Medicine (miscellaneous) Meniscus (anatomy) Menisci Tibial Article Biomaterials Sepharose chemistry.chemical_compound Chondrocytes Tissue engineering Tensile Strength Materials Testing Ultimate tensile strength medicine Surface roughness Animals Glycosaminoglycans Models Statistical Tissue Engineering Cartilage Anatomy Coculture Techniques Phenotype medicine.anatomical_structure chemistry Agarose Collagen Rabbits Biomedical engineering |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 3:521-530 |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | The meniscus is a fibrocartilaginous tissue that is critically important to the loading patterns within the knee joint. If the meniscus structure is compromised, there is little chance of healing, due to limited vascularity in the inner portions of the tissue. Several tissue-engineering techniques to mimic the complex geometry of the meniscus have been employed. Of these, a self-assembly, scaffoldless approach employing agarose moulds avoids drawbacks associated with scaffold use, while still allowing the formation of robust tissue. In this experiment two factors were examined, agarose percentage and mould surface roughness, in an effort to consistently obtain constructs with adequate geometric properties. Co-cultures of ACs and MCs (50:50 ratio) were cultured in smooth or rough moulds composed of 1% or 2% agarose for 4 weeks. Morphological results showed that constructs formed in 1% agarose moulds, particularly smooth moulds, were able to maintain their shape over the 4 week culture period. Significant increases were observed for the collagen II:collagen I ratio, total collagen, GAG and tensile and compressive properties in smooth wells. Cell number per construct was higher in the rough wells. Overall, it was observed that the topology of an agarose surface may be able to affect the phenotypic properties of cells that are on that surface, with smooth surfaces supporting a more chondrocytic phenotype. In addition, wells made from 1% agarose were able to prevent construct buckling potentially, due to their higher compliance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|