Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation
| Autor: | Terren K. Niethamer, Audrey K. O’Neill, Jeffrey O. Bush, Andrew R. Larson, Hsin-Yi Henry Ho, Abigail A. Kindberg, Michael E. Greenberg |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Cell Neuroepithelial Cells Cell Count Medical and Health Sciences Mice Models Receptors 2.1 Biological and endogenous factors Aetiology Research Articles rho-Associated Kinases Eph Family Cell Differentiation Actomyosin Biological Sciences Cell sorting Cell biology medicine.anatomical_structure Embryo embryonic structures Signal transduction Signal Transduction animal structures 1.1 Normal biological development and functioning Green Fluorescent Proteins Ephrin-B1 Biology Models Biological Article 03 medical and health sciences Underpinning research medicine Animals Humans Ephrin Actin Receptors Eph Family Mammalian HEK 293 cells Erythropoietin-producing hepatocellular (Eph) receptor Cell Biology Embryo Mammalian Biological Actins biological factors HEK293 Cells 030104 developmental biology Generic health relevance sense organs Developmental Biology |
| Zdroj: | The Journal of cell biology, vol 215, iss 2 The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201604097 |
| Popis: | Eph/ephrins drive cell segregation and boundary formation. O’Neill et al. discover that segregation is driven by unidirectional kinase-dependent EphB signaling. Unidirectional signaling generates a cortical actin differential between ephrin-B1– and EphB2-expressing cells and requires ROCK activity for cell segregation. Cell segregation is the process by which cells self-organize to establish developmental boundaries, an essential step in tissue formation. Cell segregation is a common outcome of Eph/ephrin signaling, but the mechanisms remain unclear. In craniofrontonasal syndrome, X-linked mosaicism for ephrin-B1 expression has been hypothesized to lead to aberrant Eph/ephrin-mediated cell segregation. Here, we use mouse genetics to exploit mosaicism to study cell segregation in the mammalian embryo and integrate live-cell imaging to examine the underlying cellular and molecular mechanisms. Our data demonstrate that dramatic ephrin-B1–mediated cell segregation occurs in the early neuroepithelium. In contrast to the paradigm that repulsive bidirectional signaling drives cell segregation, unidirectional EphB kinase signaling leads to cell sorting by the Rho kinase–dependent generation of a cortical actin differential between ephrin-B1– and EphB-expressing cells. These results define mechanisms of Eph/ephrin-mediated cell segregation, implicating unidirectional regulation of cortical actomyosin contractility as a key effector of this fundamental process. |
| Databáze: | OpenAIRE |
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