Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays forTaenia soliumcysticercosis
| Autor: | Hector H. Garcia, Louis Jacob, Patricia P. Wilkins, Yesenia Castillo, Javier A. Bustos, Robert H. Gilman, Armando E. Gonzalez, Oscar H. Del Brutto, Isidro Gonzales, Herbert Saavedra |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hymenolepiasis 030106 microbiology 030231 tropical medicine Neurocysticercosis Heterologous Enzyme-Linked Immunosorbent Assay Immunologic Tests Sensitivity and Specificity Article Serology 03 medical and health sciences 0302 clinical medicine Antigen antibody Peru Taenia solium parasitic diseases Animals Medicine neurocysticercose biology Immunomagnetic Separation business.industry cysticercosis Public Health Environmental and Occupational Health neurocysticercosis Cysticercosis medicine.disease Virology medicine.drug_formulation_ingredient Infectious Diseases cysticercose Antigens Helminth anticorps Pérou biology.protein ELISA Parasitology Antibody business purl.org/pe-repo/ocde/ford#3.03.06 [https] |
| Zdroj: | Tropical Medicine & International Health. 23:101-105 |
| ISSN: | 1360-2276 |
| DOI: | 10.1111/tmi.13010 |
| Popis: | Objective To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen® , for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen® . All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis. |
| Databáze: | OpenAIRE |
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