Cyclic Hypoxia Conditioning Alters the Content of Myoblast-Derived Extracellular Vesicles and Enhances Their Cell-Protective Functions
| Autor: | IJu Lo, Marie Vognstoft Hjortbak, Stine Duelund Kaas Christensen, Tingting Gu, Kim Ryun Drasbek, Hans Erik Bøtker, Jørgen Kjems, Ping Song, Andrea E. Tóth, Susanne T. Venø, Morten Nielsen, Yan Yan, Blagoy Blagoev, Thomas Ravn Lassen, Junyi Su |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Myoblast
QH301-705.5 Cell Medicine (miscellaneous) Inflammation cyclic hypoxia-reoxygenation Cyclic hypoxia-reoxygenation General Biochemistry Genetics and Molecular Biology Article In vivo medicine Myocyte Viability assay Biology (General) Tube formation remote ischemic conditioning Chemistry Remote ischemic conditioning Proteins Skeletal muscle Extracellular vesicles proteins Cell biology microRNAs MicroRNAs medicine.anatomical_structure myoblast medicine.symptom extracellular vesicles C2C12 |
| Zdroj: | Biomedicines Volume 9 Issue 9 Yan, Y, Gu, T, Christensen, S D K, Su, J, Lassen, T R, Hjortbak, M V, Lo, IJ, Venø, S T, Tóth, A E, Song, P, Nielsen, M S, Bøtker, H E, Blagoev, B, Drasbek, K R & Kjems, J 2021, ' Cyclic Hypoxia Conditioning Alters the Content of Myoblast-Derived Extracellular Vesicles and Enhances Their Cell-Protective Functions ', Biomedicines, vol. 9, no. 9 . https://doi.org/10.3390/biomedicines9091211 Biomedicines, Vol 9, Iss 1211, p 1211 (2021) Yan, Y, Gu, T, Christensen, S D K, Su, J, Lassen, T R, Hjortbak, M V, Lo, I, Venø, S T, Tóth, A E, Song, P, Nielsen, M S, Bøtker, H E, Blagoev, B, Drasbek, K R & Kjems, J 2021, ' Cyclic hypoxia conditioning alters the content of myoblast-derived extracellular vesicles and enhances their cell-protective functions ', Biomedicines, vol. 9, no. 9, 1211 . https://doi.org/10.3390/biomedicines9091211 |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines9091211 |
| Popis: | Remote ischemic conditioning (RIC) is a procedure that can attenuate ischemic-reperfusion injury by conducting brief cycles of ischemia and reperfusion in the arm or leg. Extracellular vesicles (EVs) circulating in the bloodstream can release their content into recipient cells to confer protective function on ischemia-reperfusion injured (IRI) organs. Skeletal muscle cells are potential candidates to release EVs as a protective signal during RIC. In this study, we used C2C12 cells as a model system and performed cyclic hypoxia-reoxygenation (HR) to mimic RIC. EVs were collected and subjected to small RNA profiling and proteomics. HR induced a distinct shift in the miRNA profile and protein content in EVs. HR EV treatment restored cell viability, dampened inflammation, and enhanced tube formation in in vitro assays. In vivo, HR EVs showed increased accumulation in the ischemic brain compared to EVs secreted from normoxic culture (N EVs) in a mouse undergoing transient middle cerebral artery occlusion (tMCAO). We conclude that HR conditioning changes the miRNA and protein profile in EVs released by C2C12 cells and enhances the protective signal in the EVs to recipient cells in vitro. |
| Databáze: | OpenAIRE |
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