Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli
| Autor: | Wilfried Wackernagel, Stefan Graupner |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Expression vector
Ecology biology biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Enterobacteriaceae Pichia Microbiology law.invention Transformation (genetics) Plasmid Restriction map law Escherichia coli medicine Recombinant DNA Transformation Bacterial Plasmids Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 62:1839-1841 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.62.5.1839-1841.1996 |
| Popis: | Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study. |
| Databáze: | OpenAIRE |
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