Accurate interrogation of FCGR3A rs396991 in European and Asian populations using a widely available TaqMan genotyping method
| Autor: | Heather A. Niederer, Sarah M. Glass, Elizabeth C. Harris, Kay E. Murphy, Karen S. King, Charles J. Cox |
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| Rok vydání: | 2015 |
| Předmět: |
Heterozygote
Genotype Genotyping Techniques FCGR3A Short Communication TaqMan Biology Polymerase Chain Reaction Polymorphism Single Nucleotide White People law.invention symbols.namesake Asian People law Genetics Humans General Pharmacology Toxicology and Pharmaceutics Molecular Biology Genotyping Genetics (clinical) Polymerase chain reaction Sanger sequencing Homozygote Receptors IgG Sequence Analysis DNA FCGR3B rs396991 Amino Acid Substitution genotyping Fc gamma receptor IIIa ComputingMethodologies_DOCUMENTANDTEXTPROCESSING symbols Molecular Medicine F176V |
| Zdroj: | Pharmacogenetics and Genomics |
| ISSN: | 1744-6872 |
| DOI: | 10.1097/fpc.0000000000000175 |
| Popis: | Supplemental Digital Content is available in the text. A polymorphism in the receptor for the Fc region of IgG, Fc γ-receptor IIIa (FcγRIIIa, FCGR3A rs396991), has been inconsistently shown in the literature to have an effect on response to monoclonal antibody therapy in several indications. The rs396991 (T/G) polymorphism leads to an F176V substitution and increased affinity for IgG. This variant has proven difficult to genotype accurately, primarily because of extensive homology between the FCGR3A and FCGR3B genes. We have shown that rs396991 can be genotyped by PCR amplification, followed by direct Sanger sequencing of the product, without coamplification of FCGR3B, and that the rs396991 TaqMan assay (C__25815666_10) agrees with Sanger sequencing results in 100% of European and Asian samples tested, but it has a small error rate in African and American populations. C__25815666_10 is therefore suitable to interrogate rs396991 in studies involving Europeans and Asians; however for other populations, the default genotyping method should be PCR followed by Sanger sequencing. |
| Databáze: | OpenAIRE |
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