Enterococcus faecalis aggravates pyelonephritis caused by Pseudomonas aeruginosa in experimental ascending mixed urinary tract infection in mice
| Autor: | Toshiyuki Yamazaki, K Okonogi, N Tsuchimori, Ryogo Hayashi, A Shino |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Urinary system Immunology Antibiotics Kidney medicine.disease_cause Microbiology Enterococcus faecalis Mice medicine Renal medulla Animals Antigens Bacterial Pyelonephritis biology Pseudomonas aeruginosa biology.organism_classification Streptococcaceae Infectious Diseases medicine.anatomical_structure Urinary Tract Infections Mice Inbred CBA Female Parasitology Renal pelvis Research Article |
| Zdroj: | Infection and Immunity. 62:4534-4541 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.62.10.4534-4541.1994 |
| Popis: | Mixed urinary tract infection was caused by simultaneous inoculation of 10(4) CFU each of Enterococcus faecalis TN2005 and Pseudomonas aeruginosa P9 into the bladders of CBA/J mice. Both organisms proliferated in the kidneys, and viable cell counts of E. faecalis TN2005 reached a peak level of 4.1 x 10(5) CFU per pair of kidneys within the first 24 h, while P. aeruginosa P9 counts increased more slowly. The number of P. aeruginosa P9 cells peaked at 8.3 x 10(6) CFU per pair of kidneys 5 days after infection. Five days after mixed infection, infiltration of neutrophils into the renal pelvis and renal medulla was observed. Immunohistochemical staining revealed the presence of E. faecalis antigen in the renal medulla. P. aeruginosa antigen was detected mainly in the renal pelvis 5 days after infection and in the renal medulla as well as the renal pelvis 14 days after infection. Mixed infection induced pyelonephritis within 5 days after mixed infection, while it was not observed until 14 days after infection with P. aeruginosa P9 alone. P. aeruginosa P9 inoculated together with E. faecalis TN2005 was more resistant to eradication from the kidneys by beta-lactam antibiotics than P. aeruginosa P9 inoculated alone. These results suggest that E. faecalis TN2005 invades the renal medulla first in mixed urinary tract infection and induces histological changes which lead to aggravation of the pyelonephritis caused by P. aeruginosa P9. |
| Databáze: | OpenAIRE |
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