Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Autor: Yu-Ting Gao, Liu Huimin, Liu Qinglei, Wei Wang, Ji-Wen Wei, Yin-Feng Chen
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Antioxidant
medicine.medical_treatment
Pharmaceutical Science
Organic chemistry
antioxidant activity
passion fruit
Cellulase
01 natural sciences
High-performance liquid chromatography
Article
Antioxidants
Analytical Chemistry
ultrasonic cellulase extraction
chemistry.chemical_compound
0404 agricultural biotechnology
QD241-441
Drug Discovery
medicine
Phenol
Ultrasonics
Response surface methodology
Physical and Theoretical Chemistry
Chromatography
High Pressure Liquid
ABTS
Chromatography
biology
Box-Behnken Design (BBD)
antioxidant activity
Passiflora
Plant Extracts
010401 analytical chemistry
Extraction (chemistry)
Polyphenols
food and beverages
04 agricultural and veterinary sciences
040401 food science
0104 chemical sciences
polyphenol
chemistry
Chemistry (miscellaneous)
Polyphenol
biology.protein
Molecular Medicine
Box-Behnken Design (BBD)
Zdroj: Molecules
Volume 26
Issue 9
Molecules, Vol 26, Iss 2494, p 2494 (2021)
ISSN: 1420-3049
DOI: 10.3390/molecules26092494
Popis: In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje