Tomato Apical Leaf Curl Virus: A Novel, Monopartite Geminivirus Detected in Tomatoes in Argentina
| Autor: | Elin Teppa, Veronica Andrea Bornancini, Carlos Gaston Vaghi Medina, Paola Maria Lopez Lambertini, Cristina Marino-Buslje, Ceferino Rene Flores |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) TREEHOPPER food.ingredient TOALCV Argentina lcsh:QR1-502 RECOMBINATION Biology Topocuvirus tomato Microbiology lcsh:Microbiology Ciencias Biológicas SEQUENCE SIMILARITY NETWORK purl.org/becyt/ford/1 [https] 03 medical and health sciences food Solanum lycopersicum Tomate Plant virus Curtovirus Geminiviridae purl.org/becyt/ford/1.6 [https] Gene Original Research Genetics ARGENTINA Phylogenetic tree Coat Proteins food and beverages treehopper ToALCV specificity-determining positions biology.organism_classification recombination COAT PROTEIN SPECIFICITY-DETERMINING POSITIONS 030104 developmental biology coat protein Becurtovirus TOMATO Leaf curl Virología sequence similarity network CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Frontiers in Microbiology, Vol 8 (2018) BASE-Bielefeld Academic Search Engine Frontiers in Microbiology CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Microbiology January 2018 INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| DOI: | 10.3389/fmicb.2017.02665/full |
| Popis: | Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family. Fil: Vaghi Medina, Carlos Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina Fil: Teppa, Roxana Elin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Bornancini, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Flores, Ceferino René. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Marino, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: López Lambertini, Paola María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina |
| Databáze: | OpenAIRE |
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