Simultaneous determination of novel β ‐lactamase inhibitor WCK 4234 and Meropenem in dog plasma by LC–MS/MS and its application to preclinical pharmacokinetic study

Autor:	Kiran Patil, Amin Pathan, Sukanta Naik, Vineet Zope, Rajesh Chavan, Ravindra Yeole
Rok vydání:	2022
Předmět:	Pharmacology Clinical Biochemistry Reproducibility of Results Meropenem General Medicine Biochemistry Analytical Chemistry Cyclooctanes Dogs Tandem Mass Spectrometry Drug Discovery Animals beta-Lactamase Inhibitors Azabicyclo Compounds Molecular Biology Chromatography Liquid
Zdroj:	Biomedical Chromatography. 36
ISSN:	1099-0801 0269-3879
Popis:	A precise and accurate liquid chromatography-tandem mass spectrometric (LC-MS/MS) bioanalytical method has been developed and validated for the simultaneous quantification of WCK 4234 and meropenem (MEM) in dog plasma. Protein precipitation using acetonitrile was employed as a sample preparation approach. Cefepime was used as an internal standard. The developed method was selective, sensitive (limit of quantification, 0.075 μg/ml for both drugs), accurate (recovery90%), precise (CV 10%) and linear (r
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::319b806b55a78f281c33ba3278d8bdd7 https://doi.org/10.1002/bmc.5377 Zobrazit plný text záznamu Plný text