Cell-mediated immune response to high-passage Borrelia spirochetes in C57bl/6 mice is strictly dependent on antigen specificity
| Autor: | Tadej Malovrh, Igor Gruntar, Mojca Škoberne, Vladimir Kotnik |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cellular immunity
Time Factors Dose-Response Relationship Immunologic Spirochaetaceae Borrelia afzelii medicine.disease_cause Lymphocyte Activation Microbiology Mice Antigen Species Specificity Borrelia parasitic diseases medicine Animals Borrelia burgdorferi Antigens Bacterial Immunity Cellular biology bacterial infections and mycoses biology.organism_classification Antibodies Bacterial Mice Inbred C57BL Polyclonal antibodies biology.protein Borrelia garinii Borrelia Infections Spleen |
| Zdroj: | Microbiological Research. 155(4):331-338 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/s0944-5013(01)80012-4 |
| Popis: | Inbred C57bl/6 mice were challenged with high-passage Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto and tested for antigen specific T-cell response in vitro. Sonicated preparations of washed spirochetes were potent cell activators, capable of stimulating polyclonal proliferation after 72h of culture while increasing the incubation time up to 120h provoked specific cell-mediated response. Isolated murine spleocytes previously sensitized to B. burgdorferi sensu lato but not those from control mice could be induced for antigen-specific proliferation in vitro, as revealed by [3H]thymidine incorporation assay, Moreover, in mice presensitized to B. burgdorferi sensu lato, detectable cell-mediated response could be induced only with antigen preparations derived from a corresponding strain but not with those obtained from other Borrelia genospecies. The current study emphasises that the B. burgdorferi antigen-specific response may also be expected in different genospecies infections in men. |
| Databáze: | OpenAIRE |
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