Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
| Autor: | Jeong Yoon Park, Sung Uk Kuh, H.S. Park, Young Sul Yoon |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
musculoskeletal diseases medicine.medical_treatment Osteocalcin Type II collagen Bone Morphogenetic Protein 2 Fluorescent Antibody Technique Intervertebral Disc Degeneration Lumbar vertebrae Collagen Type I Degenerative disc disease Transforming Growth Factor beta1 Andrology Lumbar Transforming Growth Factor beta Genetics medicine Humans Aggrecans Intervertebral Disc Collagen Type II Molecular Biology Cells Cultured Aged Lumbar Vertebrae biology Reverse Transcriptase Polymerase Chain Reaction Chemistry SOX9 Transcription Factor General Medicine Middle Aged Alkaline Phosphatase medicine.disease Recombinant Proteins Cytokine medicine.anatomical_structure Gene Expression Regulation Microscopy Fluorescence Cervical Vertebrae biology.protein Cytokines Alkaline phosphatase Type I collagen |
| Zdroj: | Genetics and Molecular Research. 12:838-851 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/2013.march.15.4 |
| Popis: | We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II collagen, osteocalcin, and Sox9 in the 2 groups were compared by real-time PCR, before and following treatment with rhBMP-2 and TGF-β1. Immunoreactivity was analyzed to check protein activity. Type I collagen expression was significantly higher in cervical compared with that in lumbar disc cells. The mRNA expression was significantly increased after rhBMP-2 and TGF-β1 treatment. After rhBMP-2 treatment, mRNA expression of type I and II collagens increased significantly more in cervical than in lumbar NP cells. Following TGF-β1 treatment, the increase in mRNA expression was not significantly different between cervical and lumbar disc cells. Protein immunoreactivity, before and after cytokine treatment was similar to mRNA expression data. The matrix-related gene expression of cervical and lumbar NP after rhBMP-2 and TGF-β1 treatment increased similarly, with the exception of collagen expression. |
| Databáze: | OpenAIRE |
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