Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids
| Autor: | Tamara Ecem Korkut, Ezgi Rana Temel, Ezel Boyacı, Kubra Kahremanoglu, Caner Durucan, Mürvet Volkan, Atakan Arda Nalbant, Bersu Baştuğ Azer |
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| Rok vydání: | 2020 |
| Předmět: |
Analyte
Filtration and Separation Context (language use) medicine.disease_cause Solid-phase microextraction 01 natural sciences Analytical Chemistry 03 medical and health sciences Sample volume Tandem Mass Spectrometry Lc ms ms medicine Humans Sample preparation Chromatography High Pressure Liquid Solid Phase Microextraction 030304 developmental biology Detection limit 0303 health sciences Chromatography Deoxyadenosines Chemistry 010401 analytical chemistry Healthy Volunteers 0104 chemical sciences Body Fluids Oxidative Stress 8-Hydroxy-2'-Deoxyguanosine Oxidative stress Biomarkers |
| Zdroj: | Journal of separation scienceREFERENCES. 43(9-10) |
| ISSN: | 1615-9314 |
| Popis: | Recently the connection between oxidative stress and various diseases, including cancer and Alzheimer's, attracts notice as a pathway suitable for diagnostic purposes. 8-Oxo-deoxyguanosine and 8-oxo-deoxyadenosine produced from the interaction of reactive oxygen species with DNA become prominent as biomarkers. Several methods have been developed for their determination in biofluids, including solid-phase extraction and enzyme-linked immunosorbent assays. However, still, there is a need for reliable and fast analytical methods. In this context, solid-phase microextraction offers many advantages such as flexibility in geometry and applicable sample volume, as well as high adaptability to high-throughput sampling. In this study, a solid-phase microextraction method was developed for the determination of 8-oxo-deoxyguanosine and 8-oxo-deoxyadenosine in biofluids. The extractive phase of solid-phase microextraction consisted of hydrophilic-lipophilic balanced polymeric particles. In order to develop a solid-phase microextraction method suitable for the determination of the analytes in saliva and urine, several parameters, including desorption solvent, desorption time, sample pH, and ionic strength, were scrutinized. Analytical figures of merit indicated that the developed method provides reasonable interday and intraday precisions ( |
| Databáze: | OpenAIRE |
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