Chemogenetic activation of oxytocin neurons: Temporal dynamics, hormonal release, and behavioral consequences
| Autor: | Diego Benusiglio, Sophia Probst, Valery Grinevich, Yan Tang, Thomas Grund, Ferdinand Althammer, Inga D. Neumann, Lena Oppenländer |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Microdialysis Endocrinology Diabetes and Metabolism Cell Hypothalamus Biology Oxytocin 03 medical and health sciences 0302 clinical medicine Endocrinology Muscarinic acetylcholine receptor medicine Animals Humans Premovement neuronal activity Rats Wistar Social Behavior Biological Psychiatry Neurons Behavior Animal Endocrine and Autonomic Systems Chemogenetics Receptors Muscarinic Rats 030227 psychiatry Psychiatry and Mental health medicine.anatomical_structure nervous system Receptors Oxytocin Calcium Neuroscience 030217 neurology & neurosurgery Hormone medicine.drug |
| Zdroj: | Psychoneuroendocrinology. 106:77-84 |
| ISSN: | 0306-4530 |
| Popis: | Chemogenetics provides cell type-specific remote control of neuronal activity. Here, we describe the application of chemogenetics used to specifically activate oxytocin (OT) neurons as representatives of a unique class of neuroendocrine cells. We injected recombinant adeno-associated vectors, driving the stimulatory subunit hM3Dq of a modified human muscarinic receptor into the rat hypothalamus to achieve cell type-specific expression in OT neurons. As chemogenetic activation of OT neurons has not been reported, we provide systematic analysis of the temporal dynamics of OT neuronal responses in vivo by monitoring calcium fluctuations in OT neurons, and intracerebral as well as peripheral release of OT. We further provide evidence for the efficiency of chemogenetic manipulation at behavioral levels, demonstrating that evoked activation of OT neurons leads to social motivation and anxiolysis. Altogether, our results will be profitable for researchers working on the physiology of neuroendocrine systems, peptidergic modulation of behaviors and translational psychiatry. |
| Databáze: | OpenAIRE |
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