Photoelectrochemical Detection of Dengue-Related Oligonucleotide Sequence using Anthocyanin as an Intercalating Agent and Electrochromic Material
| Autor: | Kusmardi Kusmardi, Aryo Tedjo, Anwar S. Ibrahim |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Photocurrent
lcsh:R5-920 Oligonucleotide lcsh:Public aspects of medicine Hybridization probe lcsh:R lcsh:Medicine lcsh:RA1-1270 Sequence (biology) Biology Dengue virus anthocyanin extract medicine.disease_cause Molecular biology Combinatorial chemistry chemistry.chemical_compound chemistry Electrochromism medicine A-DNA photoelectrochemical lcsh:Medicine (General) dengue-related oligonucleotide DNA |
| Zdroj: | Makara Journal of Health Research, Vol 14, Iss 2, Pp 70-74 (2011) Makara Seri Kesehatan, Vol 14, Iss 2, Pp 70-74 (2010) |
| ISSN: | 2356-3656 2356-3664 |
| DOI: | 10.7454/msk.v14i2.686 |
| Popis: | World Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria, i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is to detect oligonucleotide sequence using a DNA amplification technique. In this research, the measurement of dengue-related oligonucleotide was conducted by photoelectrochemical method. The presence of oligonucleotide sequence in target DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound to electrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent is directly proportional to the number of target DNA. The aim of this research is to obtain photoelectrochemical system that has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially the applicability of anthocyanin as a electrochromic substance and intercalating agent. Linearity (R2) generated from the change of current in response to concentration changes of target DNA (in the concentration range of 0.75–3.00 nM) is 0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample. |
| Databáze: | OpenAIRE |
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