Iodothyronine-phospholipid interactions in the lipid gel phase probed by Raman spectral markers
| Autor: | Rosa María Susana Álvarez, Ariel Alcides Petruk, Marcelo Clemente Sosa Morales |
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| Rok vydání: | 2012 |
| Předmět: |
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2-Dipalmitoylphosphatidylcholine Físico-Química Ciencia de los Polímeros Electroquímica Phospholipid Spectrum Analysis Raman Spectral line Analytical Chemistry chemistry.chemical_compound symbols.namesake Phase (matter) Thyronines Methylene Instrumentation THYROID HORMONES Spectroscopy Bilayer Ciencias Químicas LIPID GEL PHASE Atomic and Molecular Physics and Optics Crystallography Thyroxine DPPC LIPOSOMES Membrane chemistry Liposomes RAMAN SPECTROSCOPY symbols Polar lipids (amino acids peptides and proteins) Raman spectroscopy CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 112 |
| ISSN: | 1873-3557 |
| Popis: | A better understanding of the structural effects induced by thyroid hormones in model membranes is attained by Raman spectroscopy. The interactions of T3 and T4 with multilamellar vesicles of dipalmytoylphosphatidylcholine (DPPC) in the gel phase are characterized by analyzing the spectral behavior of the C–H and C–C stretching vibrations of the acyl chains. The spectra evidence an increase in the relative number of gauche conformation, which indicates the hormones are able to penetrate into the hydrophobic region of the bilayer and partially alter the lipid structure. In addition, the density packing of the acyl chains appears increased and the rotational mobility of the terminal methylene groups is slightly reduced in the iodothyronine/DPPC mixtures. These effects are interpreted in terms of the transition to an interdigitated phase due to the hormone incorporation to the membrane. The polar heads of the lipids also interact with the hormone, as evidenced by the PO 2 symmetric stretching band. Fil: Petruk, Ariel Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina |
| Databáze: | OpenAIRE |
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