[A rapid method for serodiagnosis of Japanese encephalitis using persistently infected C6/J-121 cells]
| Autor: | Yoshiichi Minekawa, Toru Otake, Tomoaki Kimura, Noboru Ueba |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Encephalitis Virus
Japanese biology business.industry Igm antibody Swine Persistently infected Fluorescent Antibody Technique General Medicine Japanese encephalitis medicine.disease Antibodies Viral Virology Virus Serology Specific antibody Predictive Value of Tests medicine biology.protein Animals Humans Antibody business Encephalitis Japanese Cells Cultured Antibody detection |
| Zdroj: | Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 64(3) |
| ISSN: | 0387-5911 |
| Popis: | The usefulness of persistently infected C6/36 (C6/J-121) cells with Japanese encephalitis virus (JEV) for a rapid serodiagnostic test was examined with the sera of men, swine and laboratory animals by indirect immunofluorescent antibody (IFA) technique. Detection of specific antibodies was completed within 3 to 5 hours. Nonspecific fluorescence frequently observed in other IFA tests was few. The sensitivity of antibody detection and the serological specificity of IFA (IgG) were similar to those of hemagglutination-inhibition (HI) test. For the detection of IgM antibodies in sera of JE-patients and naturally JEV infected swine, this method was found to be simpler and more sensitive and rapid than the conventional HI test which required 2-ME treatment of the sera. |
| Databáze: | OpenAIRE |
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