A homogeneous polysaccharide from Lycium barbarum: Structural characterizations, anti-obesity effects and impacts on gut microbiota
| Autor: | Hong Yin, Man Wu, Zuzhe Kang, Yifan Chang, Yi Wu, Hairong Liu, Yu Yang, Qishan Liu, Jinyou Duan |
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| Rok vydání: | 2020 |
| Předmět: |
Blood Glucose
Male Flora medicine.medical_treatment Metabolite 02 engineering and technology Gut flora Polysaccharide Biochemistry Rhamnose 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship Structural Biology In vivo medicine Animals Food science Obesity Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Bacteria Molecular Structure Chemistry Prebiotic Fatty Acids Galactose General Medicine Metabolism 021001 nanoscience & nanotechnology biology.organism_classification Arabinose Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal Prebiotics Dysbiosis Lycium Anti-Obesity Agents 0210 nano-technology Energy Metabolism Biomarkers Drugs Chinese Herbal |
| Zdroj: | International journal of biological macromolecules. 183 |
| ISSN: | 1879-0003 |
| Popis: | Lycium barbarum polysaccharides (LBPs) are known for their beneficial effects on diabetes, NAFLD and related chronic metabolic diseases induced by high-fat diet (HFD). However, the relevant researches are mainly about the whole crude polysaccharides, the specific active ingredient of LBPs and its bioactivity have been rarely explored. Herein, a homogeneous polysaccharide (LBP-W) was isolated and purified from crude LBPs. Structure characterizations indicated that LBP-W contained a main chain consisting of a repeated unit of →6)-β-Galp(1 → residues with branches composed of α-Araf, β-Galp and α-Rhap residues at position C-3. The objective of this study was to evaluate the anti-obesogenic effect of LBP-W and figure out the underlying mechanisms. In vivo efficacy trial illustrated that LBP-W supplements can alleviate HFD-induced mice obesity significantly. Gut microbiota analysis showed that LBP-W not only improved community diversity of intestinal flora, but also regulated their specific genera. Moreover, LBP-W can increase the content of short-chain fatty acids (SCFAs), a metabolite of the intestinal flora. In summary, all these results demonstrated that the homogeneous polysaccharide purified from L. barbarum could be used as a prebiotic agent to improve obesity by modulating the composition of intestinal flora and the metabolism of SCFAs. |
| Databáze: | OpenAIRE |
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