Development of reverse transcription-competitive polymerase chain reaction method to quantitate the expression levels of human sodium iodide symporter
| Autor: | Kwon-Yul Ryu, Allan Siperstein, Quan-Yang Duh, Mariwil G. Wong, Patricia A. Smanik, Sissy M. Jhiang, Orlo H. Clark, Molly E. Senokozlieff, Ernest L. Mazzaferri |
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| Rok vydání: | 1999 |
| Předmět: |
Sodium-iodide symporter
Adenoma Adult endocrine system endocrine system diseases Endocrinology Diabetes and Metabolism Thyroid Gland Thyroid carcinoma Endocrinology Reference Values medicine Humans RNA Messenger Thyroid Neoplasms Aged Messenger RNA Symporters Chemistry Reverse Transcriptase Polymerase Chain Reaction Thyroid RNA Membrane Proteins Middle Aged medicine.disease Molecular biology humanities Reverse transcriptase Carcinoma Papillary medicine.anatomical_structure Symporter Carrier Proteins |
| Zdroj: | Thyroid : official journal of the American Thyroid Association. 9(4) |
| ISSN: | 1050-7256 |
| Popis: | The sodium iodide symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To investigate whether human NIS (hNIS) mRNA levels in papillary thyroid carcinomas (PCs) correlate with the ability of tumors to concentrate radioiodide, we developed a reverse transcription-competitive polymerase chain reaction (RT-cPCR) method to quantify the hNIS mRNA levels in thyroid tissues. We studied 7 normal thyroid tissues, 8 PCs, and 1 follicular adenoma. hNIS mRNA levels in PCs were generally lower than those found in normal thyroid tissues. The reduced radioiodide concentrating activity of PCs is due, at least in part, to the reduced expression and/or the decreased stability of hNIS mRNA. |
| Databáze: | OpenAIRE |
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