Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging
| Autor: | Elise K. Kronquist, Beth A. Kozel, Leah M. Gober, Joseph R. Sukinik, Danielle R. Donahue, Russell H Knutsen, Sean E. McLean, Mark D. Levin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
General Chemical Engineering Pulmonary Artery Vascular architecture Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine medicine.artery Animals Medicine Micro ct Lung General Immunology and Microbiology business.industry Microcirculation General Neuroscience Vascular casting X-Ray Microtomography Mice Inbred C57BL Catheter 030104 developmental biology medicine.anatomical_structure Animals Newborn 030220 oncology & carcinogenesis Pulmonary artery business Catheter placement Perfusion Biomedical engineering |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| DOI: | 10.3791/61242 |
| Popis: | Blood vessels form intricate networks in 3-dimensional space. Consequently, it is difficult to visually appreciate how vascular networks interact and behave by observing the surface of a tissue. This method provides a means to visualize the complex 3-dimensional vascular architecture of the lung. To accomplish this, a catheter is inserted into the pulmonary artery and the vasculature is simultaneously flushed of blood and chemically dilated to limit resistance. Lungs are then inflated through the trachea at a standard pressure and the polymer compound is infused into the vascular bed at a standard flow rate. Once the entire arterial network is filled and allowed to cure, the lung vasculature may be visualized directly or imaged on a micro-CT (µCT) scanner. When performed successfully, one can appreciate the pulmonary arterial network in mice ranging from early postnatal ages to adults. Additionally, while demonstrated in the pulmonary arterial bed, this method can be applied to any vascular bed with optimized catheter placement and endpoints. |
| Databáze: | OpenAIRE |
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