Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol
| Autor: | Jean Jakoncic, Bruce J. Hrnjez, Sergey V. Buldyrev, Neer Asherie, Dahniel Sastow, Jerome M. Karp, Ariel Axelbaum, Jacob Berger, Mark Stauber |
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| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
precipitants Stereochemistry chirality high-resolution protein structures 010402 general chemistry Crystallography X-Ray 01 natural sciences law.invention Crystal 03 medical and health sciences chemistry.chemical_compound Glycols Protein structure Structural Biology law Crystallization crystallization additives 030304 developmental biology 0303 health sciences organic chemicals General Medicine Research Papers 0104 chemical sciences 3. Good health Protein Structure Tertiary Crystallography chemistry 2-Methyl-2 4-pentanediol Muramidase Lysozyme Enantiomer Protein crystallization Chirality (chemistry) MPD |
| Zdroj: | Acta Crystallographica Section D: Biological Crystallography |
| ISSN: | 1399-0047 |
| Popis: | Crystallization of lysozyme with (R)-2-methyl-2,4-pentanediol produces more ordered crystals and a higher resolution protein structure than crystallization with (S)-2-methyl-2,4-pentanediol. The results suggest that chiral interactions with chiral additives are important in protein crystal formation. Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization. |
| Databáze: | OpenAIRE |
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