| Autor: |
Muneyasu Shida, Toshinori Okinaga, Takayuki Nambu, Hiroshi Maeda, Hiroaki Aso |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
World Journal of Advanced Research and Reviews. 8:154-161 |
| ISSN: |
2581-9615 |
| DOI: |
10.30574/wjarr.2020.8.3.0473 |
| Popis: |
Streptococcus mitisis a normal commensal of the human oral cavity and oropharynx. This microorganism is an opportunistic pathogen in immune compromised hosts and a cause of invasive diseases such as infective endocarditis. We isolated a matrix metalloprotease (MMP)-like protein ofS. mitis. The gene for the MMP-like protein was found on the genome sequence database ofS. mitis. The gene encodes a protein consisting of 240 amino acid residues with a conserved zinc-binding motif (HEXXHXXGXXH) among the matrix metallopeptidase family. The gene was PCR-amplified fromS. mitisATCC 49456 and cloned for construction of the recombinant protein. The recombinant MMP-like protein with a GST-tag was purified and the enzymatic activity was assessed. The recombinant protein showed approximately half the level of MMP-like activity as compared to human recombinant MMP-8. The MMP-like protein ofS. mitismay be involved in the pathogenesis of infection to the dentin with collagen fibers and systemic invasive diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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