| Popis: |
In studies on in vitro propagation of Vitis vinifera L. (cvs Chardonnay, Pinot white, Sultanina, plavac mali and Plavac mali sivi, a spontaneous variant), shoot explants, 1-2 mm long, were grown on media with different types and concentrations of growth regulators, macronutrients and carbohydrates. Excised buds from canes, collected in march and held in water and in a controlled laboratory environment, were the source of explants. Very successful surface sterilization of buds was achieved with 1, 5% Izosan (chlorine product, Pliva, Zagreb) and 0, 01 % Tween 20 for 15 min, and rinsed in sterile distilled water (3 x 5 min.) Two basal media with macronutrients according to MURASHIGE and SKOOG (MS), full and half strength, and LLOYD and McCOWN (WPM) supplemented with 2% sucrose, 0, 8% Difco bacto agar and (mgl -1): 1 thiamine: HC1, 0, 5 pyridoxine: HC1, 2 glycine, 100 myo-inositol were tested. Cytokinin BA and auxins IAA and NAA in different concentrations and combinations were studied. The full strength MS medium with 1 mgl-1 BA and 0, 3 mgl-1 IAA gave the best results in establishing multiple shoot cultures. Effects of cultivar and time length in culture on multiplication rate, leaf shape deviation and vitrification was analysed. |
