Inhibitory Effects of Olea europaea Leaf Extract on Mesenchymal Transition Mechanism in Glioblastoma Cells

Autor: Cagla Tekin, Secil Ak Aksoy, Unal Egeli, Gulsah Cecener, Melis Mutlu, Berrin Tunca
Přispěvatelé: Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı., Mutlu, Melis, Tunca, Berrin, Aksoy, Seçil, Tekin, Çağla, Egeli, Ünal, Çeçener, Gülşah, AAH-1420-2021, ABI-6078-2020, AAH-8540-2021
Rok vydání: 2020
Předmět:
0301 basic medicine
Cancer Research
Concomitant
Unclassified drug
Medicine (miscellaneous)
Expression
Antiproliferative activity
Snail
Nerve cell adhesion molecule
0302 clinical medicine
Invasion
Nutrition and Dietetics
Transition (genetics)
biology
Plant leaf
Cell migration
Wound healing assay
Transcription factor ZEB1
Cadherins
Epithelial-mesenchymal transition
Stem-cells
Cell invasion
Plant extract
Antineoplastic agent
Migration inhibition
Oncology
030220 oncology & carcinogenesis
Uvomorulin
Drug mechanism
Human
Transcription factor twist
Efficacy
Inhibitory postsynaptic potential
Methylation
Adjuvant temozolomide
Article
Association
03 medical and health sciences
Transcription factor snail
Glioblastoma cell line
Olea
biology.animal
Epithelial Mesenchymal Transition
Transcription Factor ZEB1
Metastasis
Genetics
medicine
Humans
Nutrition & dietetics
Snail family transcription factors
Transcription factor
Antineoplastic activity
030109 nutrition & dietetics
Radiotherapy
Mesenchymal stem cell
Plant extracts
Tumor cell line
Nonhuman
medicine.disease
Brain tumor
Brain neoplasms
Cell line
tumor
Human cell
Cadherin
Cancer research
Protein expression
Olea europaea extract
Glioblastoma
Epithelial mesenchymal transition
Wound healing
Olive tree
Olive oil
Zdroj: Nutrition and Cancer. 73:713-720
ISSN: 1532-7914
0163-5581
DOI: 10.1080/01635581.2020.1765260
Popis: Background: Glioblastoma (GB) is the most aggressive form of brain tumor. Despite the current treatment methods, the survival rate of patients is very low. Therefore, there is a need to develop new therapeutic agents. The migration and invasion capacity of GB cells is related to mesenchymal transition (MT) mechanism. Materials and Methods: The effect of OLE on MT was determined by analysis of the Twist, Snail, Zeb1, N-cadherin and E-cadherin genes in the EMT mechanism. The effect of OLE on cell migration was determined by wound healing test. Results: 2 mg/ml OLE reduced Twist, Snail, Zeb1 and N-cadherin expression and the combination of OLE + TMZ (2 mg/ml OLE + 350 mM TMZ) increased E-cadherin and reduced Twist, Zeb1 and N-cadherin. In addition, co-treatment with OLE increased TMZ-induced anti-invasion properties thought suppressing transcription factors of MT mechanism. Conclusion: OLE can enhance the anti-MT activities of TMZ against GB and provide strong evidence that combined treatment with OLE and TMZ has the potential to be an effective alternative approach in GB therapy.
Databáze: OpenAIRE
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