Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine
| Autor: | Ann M. Arvin, George Kemble, Nancy Bouvier, Shaye Stamatis, Alenka M. Zeman, David B. Lewis, Tyson H. Holmes, Cornelia L. Dekker, Wenwei Tu, Harry B. Greenberg, Xiao-Song He |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
CD4-Positive T-Lymphocytes Male Cellular immunity Influenza vaccine T-Lymphocytes Statistics as Topic CD8-Positive T-Lymphocytes Antibodies Viral Interferon-gamma Immune system Interferon Neutralization Tests Medicine Humans Lymphocyte Count Child biology business.industry Influenza A Virus H3N2 Subtype Age Factors Hemagglutination Inhibition Tests Flow Cytometry Virology Lymphocyte Subsets Vaccination Influenza B virus Infectious Diseases Immunization Vaccines Inactivated Influenza A virus Influenza Vaccines Child Preschool Pediatrics Perinatology and Child Health Humoral immunity Immunology biology.protein Female Antibody business medicine.drug |
| Zdroj: | The Pediatric infectious disease journal. 26(2) |
| ISSN: | 0891-3668 |
| Popis: | There have been no prior reports of the frequency of circulating influenza-specific, interferon gamma-producing memory CD4+ and CD8+ T-cells in healthy children who have received multiple influenza immunizations.We evaluated 21 previously immunized children, ages 3 to 9 years, before and 1 month after administration of trivalent inactivated influenza vaccine. Frequencies of influenza-specific CD4+ and CD8+ T-cells stimulated with trivalent inactivated influenza vaccine or A/Panama (H3N2) virus were determined by flow cytometry, and antibody responses to vaccine strains and a drifted H3N2 strain were measured by hemagglutination inhibition assay and neutralizing antibody assays.Mean change in CD4+ and in CD8+ T-cell frequencies after immunization was 0.01% (P0.39) with postimmunization CD4+ frequencies higher than CD8+ frequencies. Children with more previous vaccinations had a higher baseline frequency of CD4+ T-cells (P = 0.0002) but a smaller increase or even a decline from baseline after immunization (P = 0.003). An association between age and change in frequency was not detected. Baseline geometric mean titers (GMTs) and seroprotection rates were significantly higher in older children against A/Panama (neutralizing baseline GMT, P = 0.0488) and A/New Caledonia (hemagglutination inhibition baseline GMT and seroprotection, P0.0297). Baseline GMTs against B/Hong Kong were not associated with age or quantity of prior vaccinations.These findings suggest that children may plateau in CD4+ T-cell responses to influenza antigens with repeated exposures and that the number of exposures may play a large role in building a memory CD4+ T-cell response to influenza A, perhaps independently from age. |
| Databáze: | OpenAIRE |
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