Does enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin A subunits?
| Autor: | Erik L. Hewlett, Myron M. Levine, Richard L. Guerrant, K T Tiemens, C S Weikel, S A Long-Krug |
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| Rok vydání: | 1984 |
| Předmět: |
Diarrhea
Cholera Toxin Macromolecular Substances Bacterial Toxins Immunology Enterotoxin Biology Heat-labile enterotoxin medicine.disease_cause Microbiology Enterotoxins Escherichia coli medicine Animals Humans Heat-stable enterotoxin Secretion Enteropathogenic Escherichia coli Escherichia coli Proteins Chinese hamster ovary cell Cholera toxin Infectious Diseases Parasitology Research Article |
| Zdroj: | Infection and Immunity. 46:612-614 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.46.2.612-614.1984 |
| Popis: | Although most enteropathogenic Escherichia coli strains do not produce recognized enterotoxins, we wished to examine whether they produce any factors like heat-stable enterotoxin b or cholera toxin active subunits that might be missed by conventional assay methods. E. coli strains E851 (O142) and E2348 (O127) that had caused diarrhea in volunteers were negative for heat-labile enterotoxin and heat-stable enterotoxin a in Chinese hamster ovary cell and suckling mouse assays, failed to cause secretion in ligated small bowel loops from 6- to 8-week-old pigs after 4 to 5 h (used to show heat-stable enterotoxin b), and did not activate adenylate cyclase in pigeon erythrocyte lysates (used to demonstrate cholera toxin A subunit). We conclude that crude, unconcentrated culture filtrates and sonicates do not mimic heat-labile or heat-stable enterotoxins or cholera toxin or its A subunit and that enteropathogenic strains of E. coli probably have yet another mechanism or group of mechanisms by which they cause diarrhea. |
| Databáze: | OpenAIRE |
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