Endogenous hydrogen sulfide regulates histone demethylase JMJD3-mediated inflammatory response in LPS-stimulated macrophages and in a mouse model of LPS-induced septic shock
| Autor: | Xinhua Liu, Yi-Zhun Zhu, Di Yang, Junbo Ge, Wanwan Jia, Chenxi Xiao, Ming Qin, Si-Yu Liu, Xi-Ling Wang, Fen Long, Weijun Wu, Li-Long Pan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Jumonji Domain-Containing Histone Demethylases Lipopolysaccharide Inflammation Endogeny Stimulation Biology Biochemistry Histones Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation parasitic diseases medicine Animals Macrophage Hydrogen Sulfide Mice Knockout Pharmacology Gene knockdown Macrophages Cystathionine gamma-Lyase Shock Septic Molecular biology Cell biology Mice Inbred C57BL Disease Models Animal 030104 developmental biology Gene Expression Regulation chemistry Gene Knockdown Techniques biology.protein Demethylase medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Biochemical Pharmacology. 149:153-162 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/j.bcp.2017.10.010 |
| Popis: | Overproduction of inflammatory mediators contributes to uncontrolled inflammation during endotoxin shock. Cystathionine-γ-lyase (CSE), an enzyme involved in hydrogen sulfide (H2S) biosynthesis, has potential anti-inflammatory activity in a variety of inflammatory diseases. Jumonji domain-containing protein 3 (JMJD3), a histone 3 Lys27 (H3K27) demethylase, has been implicated in macrophage activation, but its function in CSE-mediated anti-inflammatory activities remains unknown. In the present study CSE was found to be upregulated in macrophages and mouse lipopolysaccharide (LPS) challenge models. LPS stimulation also enhanced the activation of JMJD3 and decreased H3K27me3 levels. JMJD3 knockdown upregulated H3K27me3 levels and attenuated the LPS-mediated inflammatory response. CSE knockout amplified the inflammatory cascade by increasing JMJD3 expression in septic mice. Similarly, enhanced production of inflammatory mediators by macrophages was mitigated by CSE overexpression via inhibition of JMJD3 expression. This is the first report indicating that inflammation enhanced CSE/H2S system biosynthesis, that in turn attenuated the LPS-triggered inflammatory response by regulating JMJD3 expression. Thus, the CSE/H2S system represents an epigenetic-based modification mechanism to prevent uncontrolled inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect