Methods for successful inactivation of Rift Valley fever virus in infected mosquitoes

Autor:	Edward I Patterson, Robert P. Ellis, Nicholas A. Bergren, Heather Ann Blair, Rebekah C. Kading
Rok vydání:	2020
Předmět:	0301 basic medicine Rift Valley fever virus qx_4 030106 microbiology wc_524 Select agent Biology Virology Culex 03 medical and health sciences 030104 developmental biology Vaccine strain qx_510 Trizol Chlorocebus aethiops Animals Virus Inactivation Viral rna Dna viral Vero Cells
Zdroj:	Journal of Virological Methods. 276:113794
ISSN:	0166-0934
DOI:	10.1016/j.jviromet.2019.113794
Popis:	Ensuring the successful inactivation of select agent material is critical for maintaining compliance with federal regulations and safeguarding laboratory personnel from exposure to dangerous pathogens. Rift Valley fever virus (RVFV), naturally transmitted by mosquitoes, is classified as a select agent by the CDC and USDA due to its potential to cause significant economic losses to the livestock industry and its demonstrated potential to emerge into naïve geographic areas. Herein we describe several effective inactivation procedures for RVFV infected mosquito samples. We also demonstrate the vaccine strain MP-12 can be used as an appropriate analog for inactivation testing and describe a method of validating inactivation using Amicon filters. Briefly, we show the following inactivation methods are all effective at inactivating RVFV and MP-12 by following the manufacturers'/established protocols: 4 % paraformaldehyde, Trizol LS (ThermoFisher Scientific), MagMAX™-96 Viral RNA Isolation Kit (ThermoFisher Scientific), and Mag-Bind® Viral DNA/RNA 96 Kit (Omega Bio-Tek).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3180bd8cb1c98f604823feb0fec343b6 https://doi.org/10.1016/j.jviromet.2019.113794 Zobrazit plný text záznamu Full Text from ScienceDirect