Differences in the acidic sensitivity of transient receptor potential vanilloid 1 (TRPV1) between chickens and mice
| Autor: | Fuminori Kawabata, Ruojun Liang, Shotaro Nishimura, Shoji Tabata, Yuko Kawabata |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques animal structures Calmodulin Recombinant Fusion Proteins Mutant Mutant Chimeric Proteins Biophysics TRPV1 TRPV Cation Channels Stimulus (physiology) Biochemistry Avian Proteins Mice 03 medical and health sciences Transient receptor potential channel 0302 clinical medicine Species Specificity Dorsal root ganglion Ganglia Spinal medicine Animals Humans Amino Acid Sequence Patch clamp Binding site Molecular Biology Cells Cultured Binding Sites biology Chemistry Cell Biology Hydrogen-Ion Concentration Cell biology Mice Inbred C57BL HEK293 Cells 030104 developmental biology medicine.anatomical_structure nervous system 030220 oncology & carcinogenesis biology.protein Capsaicin Chickens |
| Zdroj: | Biochemical and Biophysical Research Communications. 515:386-393 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.05.129 |
| Popis: | Chickens, one of the most important industrial animals, are a biological animal model. Here we focused on the transient receptor potential vanilloid 1 (TRPV1) to understand the pain system for acidic stimuli in chickens compared with mice. By using a whole-cell patch clamp system, we confirmed that acidic stimuli activate both chicken TRPV1 (cTRPV1) and mouse TRPV1 (mTRPV1), but the peak current of cTRPV1 is lower than that of mTRPV1, and it is difficult to desensitize cTRPV1 with an acidic stimulus compared to mTRPV1. Since the C-terminal of the calmodulin (CaM) binding site in TRPV1 was reported as one of the important structures for TRPV1 desensitization, we made chimeric cTRPV1 in which the CaM binding site of chicken is changed to that of mouse (cTRPV1-mCaM). We also compared the acidic responses of native chicken dorsal root ganglion (DRG) cells with that of mouse DRG cells. The TRPV1-mCaM results showed that the desensitization of mutant cTRPV1 was similar to that of mTRPV1, and that the basal activities of mutant cTRPV1 were significantly higher than those of cTRPV1. It was also difficult to desensitize the chicken DRG cells with an acidic stimulus, unlike the mouse DRG cells. These results suggest that there are differences in the pain transduction systems for acidic stimuli between chickens and mice that are caused by the dysfunction of the C-terminal CaM biding site of cTRPV1. These results imply that chickens repeatedly feel weak pain from an acidic stimulus, without desensitization. |
| Databáze: | OpenAIRE |
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