A beam-walking apparatus to assess behavioural impairments in MPTP-treated mice: Pharmacological validation with R-(−)-deprenyl
| Autor: | Neil Upton, Peter L. Woodhams, Helen Chapman, Martin P. Vidgeon-Hart, Marion J. Perren, Menelas N. Pangalos, Leann P. Quinn, David Virley, Kim Brackenborough |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Monoamine Oxidase Inhibitors Tyrosine 3-Monooxygenase Dopamine Substantia nigra Striatum Mice chemistry.chemical_compound Neuropharmacology Parkinsonian Disorders Predictive Value of Tests Internal medicine Neural Pathways Selegiline medicine Animals Neurotoxin Gait Disorders Neurologic Dose-Response Relationship Drug Tyrosine hydroxylase business.industry General Neuroscience MPTP Dopaminergic Brain Ethology Corpus Striatum Mice Inbred C57BL Substantia Nigra Dose–response relationship Endocrinology nervous system chemistry 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine Anesthesia business medicine.drug |
| Zdroj: | Journal of Neuroscience Methods. 164:43-49 |
| ISSN: | 0165-0270 |
| Popis: | A beam-walking apparatus has been evaluated for its ability to detect motor impairments in mice acutely treated with the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg, s.c., single or double administration). Mice subjected to MPTP lesioning showed deficits in motor performance on the beam-walking task, for up to 6 days post-MPTP administration, as compared to saline-treated controls. In addition, MPTP-treated mice were detected to have a marked depletion in striatal dopamine levels and a concomitant reduction in substantia nigra (SN) tyrosine hydroxylase (TH) immunoreactivity, at 7 days post-MPTP administration, indicative of dopaminergic neuronal loss. Pre-administration of the potent MAO-B inhibitor R-(-)-deprenyl at 3 or 10 mg/kg, 30 min, s.c, significantly inhibited the MPTP-induced reduction in SN TH-immunoreactivity, striatal dopamine depletions and impairments in mouse motor function. The data described in the present study provides further evidence that functional deficits following an acute MPTP dosing schedule in mice can be quantified and are related to nigro-striatal dopamine function. |
| Databáze: | OpenAIRE |
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