Diel Expression of Cell Cycle-Related Genes in Synchronized Cultures of Prochlorococcus sp. Strain PCC 9511
| Autor: | Frédéric Partensky, Dominique Marie, Daniel Vaulot, Julia Holtzendorff, Isabelle Mary, F. Bruyant, Wolfgang R. Hess, Stéphan Jacquet, Laurence Garczarek |
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| Přispěvatelé: | Humboldt University Of Berlin, Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Chimie Marines (LPCM), Humboldt-Universität zu Berlin |
| Rok vydání: | 2001 |
| Předmět: |
Periodicity
MESH: Periodicity MESH: Cytoskeletal Proteins Cell division Photoperiod Molecular Sequence Data Genetics and Molecular Biology Marine Biology MESH: Cell Cycle MESH: Escherichia coli Proteins Cyanobacteria MESH: Genome Bacterial Microbiology MESH: Photoperiod 03 medical and health sciences Bacterial Proteins Cell synchronization FtsZ MESH: Bacterial Proteins Molecular Biology 030304 developmental biology [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences MESH: Molecular Sequence Data biology 030306 microbiology Caulobacter crescentus Escherichia coli Proteins Cell Cycle DNA replication Membrane Proteins MESH: Marine Biology MESH: Cyanobacteria Cell cycle biology.organism_classification Molecular biology DnaA DNA-Binding Proteins Cytoskeletal Proteins Genes Bacterial biology.protein bacteria MESH: Membrane Proteins Prochlorococcus MESH: Genes Bacterial Genome Bacterial MESH: DNA-Binding Proteins |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, 2001, 183 (3), pp.915-20. ⟨10.1128/JB.183.3.915-920.2001⟩ Journal of Bacteriology, American Society for Microbiology, 2001, 183 (3), pp.915-20. ⟨10.1128/JB.183.3.915-920.2001⟩ |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.183.3.915-920.2001 |
| Popis: | The cell cycle of the chlorophyll b -possessing marine cyanobacterium Prochlorococcus is highly synchronized under natural conditions. To understand the underlying molecular mechanisms we cloned and sequenced dnaA and ftsZ , two key cell cycle-associated genes, and studied their expression. An axenic culture of Prochlorococcus sp. strain PCC 9511 was grown in a turbidostat with a 12 h–12 h light-dark cycle for 2 weeks. During the light periods, a dynamic light regimen was used in order to simulate the natural conditions found in the upper layers of the world's oceans. This treatment resulted in strong cell cycle synchronization that was monitored by flow cytometry. The steady-state mRNA levels of dnaA and ftsZ were monitored at 4-h intervals during four consecutive division cycles. Both genes exhibited clear diel expression patterns with mRNA maxima during the replication (S) phase. Western blot experiments indicated that the peak of FtsZ concentration occurred at night, i.e., at the time of cell division. Thus, the transcript accumulation of genes involved in replication and division is coordinated in Prochlorococcus sp. strain PCC 9511 and might be crucial for determining the timing of DNA replication and cell division. |
| Databáze: | OpenAIRE |
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